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KMID : 1098420070150030215
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Korean Journal of Medicinal Crop Science
2007 Volume.15 No. 3 p.215 ~ p.219
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Analytical Optimum of Ginsenosides according to the Gradient Elution of Mobile Phase in High Performance Liquid Chromatography
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Park Ji-Yeong
Lee Chung-Yeol
Won Jun-Yeon
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Abstract
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This study was conducted to analyze not only for the quality guaranteed of red ginseng but also for the minor ginsenosides. Although several studies have reported to analyze ginseng saponins, those were focused to major saponins, including 6 to 7 ginsenosides. As increase of interest in medicinal effect of ginseng products, anasis of various ginsenosides in both red and white ginseng are strongly demanded. To perform optital condition of 12 ginsenoside analysis, We controlled HPLC conditions, such as the gradient elution of the mobile phase. We found the adequate separation method for 12 ginse-nosides. The optimum condition was as following : H_2O/CH_3CN ratios were 82/18, 70/30, 55/45 and 50/50, respectively. Sol-vent flow rate was 1.00 ma/min. Column temperature was kept to 35^{circ}C. UV detector was set to 203 nm.
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KEYWORD
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ginsenoside, HPLC, gradient elution, saponin, analysis
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